Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Binding activity of N162-shFcγRIIIa-His to IgG1. The purified N162-shFcγRIIIa-His was loaded onto a column of nonfucosylated IgG1-immobilized Sepharose. The fractions of load (lane 1), flowthrough (lane 2), and elution (lane 3) were subjected to nonreducing (A) and reducing (B) 5–20% SDS–PAGE. No detectable protein was observed in the flowthrough fraction, and the whole flowthrough fraction was concentrated to apply SDS–PAGE.
