Figure 4.

Validation of the differentially expressed genes between sMCs and mMCs. (a) sMC-specific (Cma2, Mcpt4), mMC-specific (Mcpt1, Mcpt2) and MC-common markers (Fcer1a and Kit) (left panel) and six randomly selected genes (Ces3, Cnn1, Cpe, Anxa10, Ctse and Fos) (right panel) are indicated in the representative scatter correlation graphs between sMC₁ and mMC₁. The same, two-fold induction and suppression thresholds are indicated as a yellow, blue and red line, respectively. (b) The expression levels of the genes in (a) were verified by real-time RT-PCR. The values represent the ratio of relative expression levels of mMCs to sMCs, and are shown as mean ± S.D. (n = 3). The specificity of the PCR product was confirmed by gel electrophoresis and analysis of the melting temperature. The expression level of each gene was normalized to 28S ribosomal RNA.