Figure 1:
Effect of ROCK inhibitor for cyES cell repopulation after single-cell digestion.
(A) Dose-dependent effect of two kind of ROCK inhibitors Y-27632 and Fasudil. Different characters indicate statistically significant difference (P < 0.01) determined by ANOVA and Tukey–Kramer multiple comparison tests. Data represent the mean ± SD of six experiments. (B) Immunocytochemical observation of BrdU incorporation to cyES cells maintained with/without 10 µM Y-27632.