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Effect of Y-27632 treatment to cyES cell colony formation.
(A) Number of ALP-positive colonies cultured in the absence/presence of 10 µM Y-27632 without feeder cells (*P < 0.001, n = 3). (B) Number of ALP-positive colonies cultured in the absence/presence of 10 µM Y-27632 after cryopreservation by DMSO-added cryoprotectant or vitrification method using DAP213 (*P < 0.01, n = 3).
