Table 4.

Primers used for MLST

Locus	Function	Forward primer	Reverse primer	Location (a)
dinB	DNA polymerase	5'-GTT TTC CCA GTC ACG ACG TTG TAT GAG AGG TGA GCA ATG CGT A-3'	5'-TTG TGA GCG GAT AAC AAT TTC CGT AGC CCC ATC GCT TCC AG-3'	282,284 – 282,734
icdA	Isocitrate dehydrogenase	5'-GTT TTC CCA GTC ACG ACG TTG TAA TTC GCT TCC CGG AAC ATT G-3'	5'-TTG TGA GCG GAT AAC AAT TTC ATG ATC GCG TCA CCA AAY TC-3'	1,118,658 – 1,187,173
pabB	p-aminobenzoate synthase	5'-GTT TTC CCA GTC ACG ACG TTG TAA ATC CAA TAT GAC CCG CGA G-3'	5'-TTG TGA GCG GAT AAC AAT TTC GGT TCC AGT TCG TCG ATA AT-3'	1,807,273 – 1,807,740
polB	Polymerase PolII	5'-GTT TTC CCA GTC ACG ACG TTG TAG GCG GCT ATG TGA TGG ATT C-3'	5'-TTG TGA GCG GAT AAC AAT TTC GGT TGG CAT CAG AAA ACG GC-3'	65,220 – 64, 773
putP	Proline permease	5'-GTT TTC CCA GTC ACG ACG TTG TAC TGT TTA ACC CGT GGA TTG C-3'	5'-TTG TGA GCG GAT AAC AAT TTC GCA TCG GCC TCG GCA AAG CG-3'	1,074,708 – 1,075,163
trpA	Tryptophan synthase subunit A	5'-GTT TTC CCA GTC ACG ACG TTG TAG CTA CGA ATC TCT GTT TGC C-3'	5'-TTG TGA GCG GAT AAC AAT TTC GCT TTC ATC GGT TGT ACA AA-3'	1,338,658 – 1,338,098
trpB	Tryptophan synthase subunit B	5'-GTT TTC CCA GTC ACG ACG TTG TAC ACT ATA TGC TGG GCA CCG C-3'	5'-TTG TGA GCG GAT AAC AAT TTC CCT CGT GCT TTC AAA ATA TC-3'	1,339,430 – 1,338,837
uidA	Beta-glucuronidase	5'-GTT TTC CCA GTC ACG ACG TTG TAC ATT ACG GCA AAG TGT GGG TCA AT-3'	5'-TTG TGA GCG GAT AAC AAT TTC CCA TCA GCA CGT TAT CGA ATC CTT-3'	1,615,010 – 1,614,411
OF	Sequencing primer (b)	5'-GTT TTC CCA GTC ACG ACG TTG T-3'
OR	Sequencing primer (b)	5'-TTG TGA GCG GAT AAC AAT TTC-3'

(a) Based on Escherichia coli strain 536, complete genome, GenBank accession CP000247.

(b) PCR primers have the corresponding universal sequence in 5'.