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Selection of a U3Cre/FLOXIL3 cell integration library in G418. FLOXIL3 cells (4 × 106) were infected with U3Cre virus and incubated for 16 days in suspension cultures containing 10 ng/ml IL-3 and 0.6 μg/ml active G418. At various time points aliquots were plated in agar cultures containing 5 μM ganciclovir, but no IL-3, using 1 × 105 cells per ml. Colonies were counted after incubating for 10 days. Colony numbers represent the total number of colonies obtained from 106 seeded cells.
