Figure 3.

Effect of Fgf2 on CPe vesicle size and growth. Choroid plexus epithelial cells were cultured in Matrigel either in the presence or absence of Fgf2. (A) Micrographs of two vesicles, one control (upper panel) and one Fgf2-treated (7.5 ng/ml) taken at different times in culture (day 4, day 6 and day 8). (B) Graph showing the mean diameters of untreated vesicles (controls, n = 232) and vesicles treated with 0.75 (n = 63), 7.5 (n = 96) or 75 (n = 54) ng/ml Fgf2 measured on days 4, 6, 8 and 10. At all Fgf2 concentrations the vesicle diameter is significantly higher than in controls (p < 0.001). (C) Graph showing the average vesicle diameters of control vesicles (n = 96) and vesicles treated with 10 ng/ml Fgf2 from day 6 in culture (n = 110). The results were not significant. Data are means ± SEM.