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Titration of assay reagents. A) AF488-MCP-1 was serially diluted in whole blood and allowed to react at room temperature. CD14+ monocytes were examined and the Mean Equivalence of soluble fluorescence (MESF) was reported. Maximum saturation was determined to be 60–75 nM. B) Titration of CCR2 antagonist against optimum concentration (60 nM) of AF488 MCP-1
