Figure 2.

Aberrant CpG island methylation in rho⁰ cells. Representative examples of RLGS analysis are shown comparing three parental cell lines to their corresponding rho⁰ cell lines. RLGS spots of interest are shown by solid-line circles to indicate presence of the spot (lack of methylation), while dashed-line circles indicate absence of a spot (methylation). (A) MCF12A cell-line showing spots 1 and 2 exhibiting hypermethylation in the rho⁰ cell line and spot 3 exhibiting hypomethylation in the rho⁰ cell line. (B) MDA-MB-435 cell line showing spot 1 and 2 exhibiting hypermethylation in the rho⁰ cell line. (C) 143B cell line showing RLGS spot 2E58 exhibiting hypomethylation in the rho⁰ cell line. (D) Bisulfite-sequencing of 2E58 from both the 143B parental and rho⁰ cell lines. Four clones each were sequenced across a 285 bp region containing 34 CpG dinucleotides. Each horizontal line of circles represents the sequencing data from an individual clone. Each circle represents one CpG: closed circle = methylation; open circle = no methylation.