Skip to main content
. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: Ann Neurol. 2009 Jan;65(1):47–56. doi: 10.1002/ana.21523

Fig. 5.

Fig. 5

Doxycycline inhibited staurosporine-induced apoptosis in myogenic C2C12 cells. A. Myoblasts of the mouse C2C12 cell line were either untreated, treated with 100 nM staurosporine (+STS), or treated with both 100 nM staurosporine and 2μM doxycycline (+STS, +Dox) after a 3h pre-incubation with 2 μM doxycycline. Doxycycline decreased the number of abnormally shaped nuclei (indicative of apoptosis) in staurosporine-treated cultures. B. Quantitative analysis of experiments performed as in panel A demonstrated that doxycycline decreased the percentage of abnormal, apoptotic nuclei induced by staurosporine. Error bars = SE, n=4. *P < 0.05 by t-test.