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. 2009 Feb 24;3(2):e384. doi: 10.1371/journal.pntd.0000384

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Bettiol et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. PCH1; B. NT1 and C. CX1. Each compound was tested for T. cruzi growth inhibition (black curves, left axis). 50.000 NIH/3T3 cells were incubated with 50.000 β-gal T. cruzi parasites per well in the presence of each compound and β-gal activity was assayed at 4 days. Cytotoxicity of each compound on NIH/3T3 cells was also assayed (grey curve, right axis), by incubating 50.000 cells in the presence of compounds. Viability was assayed by incubating cells on day 4 with Alamar Blue for 6 h and measuring fluorescence. Data are displayed as the mean±standard deviation of triplicate wells from one of three independent experiments.