Table 1. Phenotypes of BicD phosphorylation mutants.
Mutation | MS identification | Phenotype | |
phos. | non-phos. | ||
wild type | ff | ||
S14A | + | − | ff |
S14D | ff | ||
S33A | − | + | ff |
S34A | − | + | ff |
S36A | − | + | ff |
S39A | − | + | ff |
S45A | − | + | ff |
multiple | ff | ||
S51A | − | − | ff |
S84A | − | − | ff |
S103A | + | + | ff |
S103D | ff | ||
S103F | ff | ||
S109A | + | + | ff |
S186A | + | + | ff |
S285A | + | + | ff |
S288A | + | + | ff |
S285A+S288A | ff | ||
S305 | + | + | ff |
S310A | + | + | ff |
S310D | ff | ||
S305A+S310A | ff | ||
A40V | fs, 16 nurse cells | ||
A40V+S103A | fs, 16 nurse cells | ||
A40V+S103D | lethal with few adults, 16 nurse cells | ||
A40V+S103F | ff, few egg chambers with 16 nurse cells |
Peptides containing the indicated serines were identified (+) as serine phosphorylated form (phos.) or non-phosphorylated (non-phos.), or the peptide was never identified (−). All mutants are viable, except for BicDA40V, S103D. BicDmultiple consists of the five substitutions S33A, S34N, S36N, S39A, S45A.
ff females fertile.
fs females sterile.