Fig. 4.
Berberine inhibited SMC regrowth and migration after mechanical injury. SMCs were grown to 80% confluency. We created a linear injury line at center of cultured monolayer by scraping with a sterile pipette tip. The cells were then maintained in 1% FBS-DMEM with various concentrations of berberine for 3 days. (A) Cell morphology was microphotographed. Magnification: 100×. (B) The closure of denuded area was calculated as described in Section 2.