Fig. 1.

Cloning of human and murine histidyl-tRNA synthetase (Jo-1). (A) Full length sequences encoding human or murine Jo-1 were amplified from cDNA via PCR using primers containing terminal restriction enzyme recognition sequences as indicated. Following restriction enzyme digestion of these inserts, the Jo-1 sequences were inserted into the bacterial Maltose Binding Protein expression vector pMALc2. Automated sequencing was performed to confirm proper orientation and to rule out errors introduced during PCR amplification. (B) Amino acid sequence alignment comparing murine and human Jo-1. Sequence differences are highlighted in red.