Table 2.
Group | Primers | Sequences (5′-3′)a | Design basis | References |
---|---|---|---|---|
LRR | CLRR for | TTTTCGTGTTCAACGACG | LRR domain of the tomato Cf-9 gene conferring resistance to Cladosporium fulvum | [30] |
CLRR rev | TAACGTCTATCGACTTCT | |||
NLRR for | TAGGGCCTCTTGCATCGT | LRR domain of the tobacco N gene conferring resistance to TMV | ||
NLRR rev | TATAAAAAGTGCCGGACT | |||
RLRR for | CGCAACCACTAGAGTAAC | LRR domain in the RPS2 gene conferring resistance to Pseudomonas syringae in Arabidopsis | ||
RLRR rev | ACACTGGTCCATGAGGTT | |||
XLRR for | CCGTTGGACAGGAAGGAG | LRR domain of the rice Xa21 gene conferring resistance to Xanthomonas campestris pv oryzae | ||
XLRR rev | CCCATAGACCGGACTGTT | |||
| ||||
NBS | ANo. 2 | TATAGCGGCCGCIARIGCIARIGGIARNCC | Conserved P-loop and hydrophobic NBS regions of the N and RPS2 genes from tobacco and Arabidopsis respectively | [29] |
ANo. 3 | ATATGCGGCCGCGGIGGIGTIGGIAARACNAC | |||
S1 | GGTGGGGTTGGGAAGACAACG | Hydrophobic domain and P-loop of conserved NBSs from the N and RPS2 genes from Arabidopsis and the L6 gene from flax conferring resistance to rust | [18, 31] | |
S2 | GGIGGIGTIGGIAAIACIAC | |||
AS1 | CAACGCTAGTGGCAATCC | |||
AS3 | IAGIGCIAGIGGIAGICC | |||
| ||||
PtoKin | Ptokin1 | GCATTGGAACAAGGTGAA | Serine/threonine protein kinase domain of the Pto gene conferring resistance to the bacterial pathogen Pseudomonas syringae pv tomato in tomato | [30] |
Ptokin2 | AGGGGGACCACCACGTAG | |||
Ptokin3 | TAGTTCGGACGTTTACAT | |||
Ptokin4 | AGTGTCTTGTAGGGTATC | |||
RLK for | GAYGTNAARCCIGARAA | Serine/threonine kinase sequence subdomains of the wheat Lr10 gene conferring resistance to Puccinia recondita | [20] | |
RLK rev | TCYGGYGCRATRTANCCNGGITGICC | |||
aCode for mixed bases: D = A/G/T; I = Inosine; N = A/G/C/T; R = A/G; Y = C/T.