Figure 1.

Selective regulation of host gene expression following PSbMV infection of pea cotyledonary tissues. The orientation of the pea cotyledon sections is shown in (A). B–E, F and G, H–K, and L and M were from four separate embryos. Sections B–G and H–M are from healthy and PSbMV-infected cotyledons, respectively. The sections were subjected to in situ hybridization with probes for PSbMV −ve RNA (B and H), PSbMV +ve RNA (C, F, I, and L), PsHSP71.2 RNA (D and J), polyubiquitin RNA (E and K), and PsHSC71.0 RNA (G and M). The zone of HSP70 and polyubiquitin induction correspond with the front of virus invasion and the onset of virus replication (H–K, arrows). In contrast, HSC70 expression is reduced at the front of virus invasion (L and M, white arrows).