Table 2. Characteristics of unique nucleotide signatures for 16S rDNA sequences of different Bacillus spp. and clusters of Bacillus sp.
| Bacillus spp./Clustera | Signature | Length (nts)b | Frequencyc |
| Bacillus cereus | AAAGTGGAATTCCATGTGTAGCGGTGAAAT | 30 | 151/211 |
| B. thuringiensis | ATAACATTTTGAACTGCATGGTTCGAAATT CTTTAGTGCTGAAGTTAACGCATTAAGCA | 3029 | 43/10863/108 |
| B. anthracis | No unique signature was detectable | 0/153 | |
| B. clausii | AATCCCATAAAGCCATTCTCAGTTCGGATT AAATGATTGGGGTGAAGTCGTAACAAGGTAAAACCGGAGCTAATACCGGATAATCCCTTT GCATTAGCTAGTTGGTAAGGTAACGGCTTA GTAGTGCCGAAGTTAACACATTAAGCACT | 30 30303029 | 14/39 31/3914/39 15/39 23/39 |
| B. halodurans | ATAATAAAAAGAACTGCATGGTTCTTTTTT ACCAAAGGGAGCTTGCTCCTAGAGGTTAGC | 3030 | 21/3601/36 |
| B. pumilus | AAGGTTTAGCCAATCCCACAAATCTGTTCT AAGGTTTAGCCAATCCCATAAATCTGTTCT ATAGTTCCTTGAACCGCATGGTTCAAGGAT | 303030 | 14/8336/8342/83 |
| B. megaterium | ATGATTGAAAGATGGTTTCGGCTATCACTT AATCCCATAAAACCATTCTCAGTTCGGATT AACTGATTAGAAGCTTGCTTCTATGACGTT TCTTGACATCCTCTGACAACTCTAGAGATA TGGGATAACTTCGGGAAACCGAAGCTAATA | 3030303030 | 36/4719/4728/4733/4734/47 |
| B. sphaericus | TAAAACTCTGTTGTAAGGGAAGAACAAGTA ATAGTGGAATTCCAAGTGTAGCGGTGAAAT TAATCCGATAAAGTCGTTCTCAGTTCGGAT AGTAACACGTGGGCAACCTACCTTATAGTT TAACTGGCTGTACCTTGACGGTACCTTATT | 3030303030 | 23/4214/4223/4210/4227/42 |
| B. subtili s | No unique signature was detectable | 0/211 | |
| B. licheniformis | No unique signature was detectable | 0/131 | |
| Bacillus sp. Cluster 1 | TAAAACTCTGTTGTAAGGGAAGAACAAGTA AATCCCATAAAACCGTTCCCAGTTCGGAT | 3029 | 19/4605/46 |
| Bacillus sp. Cluster 5 | AATCCCATAAATCTATTCTCAGTTCGGATT | 30 | 05/32 |
| Bacillus sp. Cluster 6 | AAGCAAATCCCATAAAACCATTCTCAGT TCAAGCAAATCCCATAAAACCATTCTCAGT | 2830 | 09/2307/23 |
| Bacillus sp. Cluster 7 | ATAACTCATTTCCTCGCATGAGGAAATGTT | 30 | 10/37 |
| Bacillus sp. Cluster 9 | AATCCCATAAAACCACTCTCAGTTCGGATT | 30 | 01/25 |
| Bacillus sp. Cluster 10 | AATCCCACAAAACCGTTCCCAGTTCGGATT | 30 | 03/25 |
| Bacillus sp. Cluster 11 | TAAACGATGAGTGCTAAGTGTTAGAGGGGT GTCGTAAAGCTCTGTTGTGAGGGACGAAGG | 3030 | 23/5025/50 |
a
Cluster represent isolates defined only up to genus level Bacillus sp.
b
nucleotides.
c
Frequency of occurrence of the signature out of the total sequences screened.
Out of 11 Clusters, no unique signatures were detectable in Clusters 2, 3, 4 and 8.