Table 4. Closest matches of Clusters of Bacillus sp. with known Bacillus sp. on the basis of restriction enzyme pattern analysis.
Bacillus sp. | Restriction enzyme | No. of Best matches | ||||||
BfaI | DpnII | RsaI | Tru9I | AluI | HaeIII | Bacillus spp. | Uniquea | |
Cluster 1 (22) b | B. sphaericus | unique | B. licheniformisB. subtilis | B. licheniformis | unique | B. sphaericusB. halodurans | 4 | 2 |
Cluster 2 (12) | B. halodurans | B. clausii | B. megaterium | unique | unique | Unique | 3 | 3 |
Cluster 3 (18) | B. halodurans | unique | B. megaterium | B. haloduransB. clausii | unique | Unique | 3 | 3 |
Cluster 4 (22) | B. megaterium | unique | B. anthracisB. cereusB. thuringiensis | B. licheniformisB. megateriumB. licheniformis | unique | B. sphaericusB. haloduransB. megaterium | 7 | 2 |
Cluster 5 (32) | B. megaterium | unique | B. sphaericusB. licheniformis | B. licheniformisB. megateriumB. sphaericus | B. megateriumunique | Unique | 3 | 3 |
Cluster 6 (7) | Unique | unique | B. megateriumB. clausii | unique | unique | Unique | 2 | 5 |
Cluster 7 (37) | B. subtilisB. megaterium | unique | B. anthracisB. cereusB. thuringiensis | B. licheniformisB. megateriumB. sphaericus | unique | B. licheniformisB. subtilisB. pumilus | 8 | 2 |
Cluster 8 (28) | B. pumilusB. megateriumB. licheniformisB. subtilis | B. sphaericus | B. megaterium | B. licheniformis B. megateriumB. sphaericus | unique | Unique | 5 | 2 |
Cluster 9 (25) | B. licheniformisB. pumilusB. megaterium | B. sphaericus | B. megateriumB. clausii | B. licheniformis B. megateriumB. sphaericus | unique | unique | 5 | 2 |
Cluster 10 (25) | B. sphaericus | unique | unique | B. licheniformisB. sphaericusB. megaterium | unique | unique | 3 | 4 |
Cluster 11 | All were designatedc |
Did not match with any of the known RE patterns of the Bacillus sp. used in this study.
Number of 16S rDNA sequences of Bacillus sp.
Cluster 11 constituted of members of known species such as: Anoxybacillus and Geobacillus.