Table 3.
van’t Hoff Enthalpy (ΔHm) and Melting Temperature (Tm) for the Polypeptides and Glycopolypeptides
17-H-3 | Cap 17-H-3 | 17-H-6 | Cap 17-H-6 | 35-H-6 | Cap 35-H-6 | |
---|---|---|---|---|---|---|
ΔHm (kcal/mol)a,b | 20.3 ± 1.9 | 19.4 ± 1.8 | 22.2 ± 1.0 | 29.2 ± 4.8 | 18.5 ± 1.8 | 20.0 ± 2.0 |
Tm (°C)c | 33.6 ± 2.3 | 38.4 ± 0.7 | 34.9 ± 0.1 | 47.8 ± 3.1 | 35.2 ± 1.4 | 38.4 ± 1.5 |
Reported errors are based on the calculated error from duplicate measurements.
The ΔHm values for all the polypeptides and the relative glycopolypeptides are not statistically different based on the results of ANOVA, Turkey’s test (p > 0.05).
The statistical comparison of Tm values using ANOVA (Turkey’s test) showed that the Cap 17-H-3 and Cap 35-H-6 do not have significantly different Tm values than their corresponding polypeptides backbones, while the Tm of Cap 17-H-6 is statistically different than that of 17-H-6 (p < 0.05) with unknown origins.