Table 1.
Frequency of Immunolabeling and In-Situ Localization of Enzymes in Synechocystis.
| Percent of protein A-gold particles |
|||
| Protein localized | Thylakoid membranes percent ± SE | Cytosol percent ± SE | Carboxysomes percent ± SE |
| CF1 | 91.8 ± 1.6 (100%) | 7.1 ± 1.6 | 1.1 ± 0.7 |
| RPI | 72.8 ± 2.1 (79.3%) | 21.3 ± 1.9 | 5.9 ± 0.8 |
| PRK | 72.2 ± 1.5 (78.6%) | 22.9 ± 1.7 | 5.0 ± 0.8 |
| RuBisCO | 46.8 ± 2.8 (50.9%) | 16.9 ± 2.3 | 36.3 ± 3.3 |
| PGK | 66.1 ± 2.9 (72%) | 30.7 ± 2.9 | 3.2 ± 1.2 |
| GAPDH | 66.1 ± 1.6 (72%) | 29.9 ± 1.9 | 4.0 ± 1.2 |
70-nm thick sections were taken on formvar-coated copper grids and immunolabeled with respective antibodies. Gold particles on thin sections of 20 different cells were counted to calculate the distribution of CF1 and Calvin cycle enzymes. The values in parenthesis indicate gold labeling percentage normalized to labeling for CF1. All the values represent mean ± SE (n = 20).