Table 2.

Linked Activities of Calvin Cycle Enzymes and Pigment Distribution among the Three Thylakoid Fractions.

Fraction	R-5-P+ADP+Pi-dependent CO2 fixation activity		R-5-P-dependent GAPDH activity		Phycocyanin		Chlorophyll
	Activity	Percent of total	Activity	Percent of total	mg/mg Chl	Percent of total	Percent of total
40-k	0.3 ± 0.1	16.0 ± 1.0	0.2 ± 0.12	21.5 ± 16.2	0.8 ± 0.2	54.3 ± 3.7	66.1 ± 6.0
90-k	2.5 ± 1.2	27.3 ± 3.7	0.99 ± 0.5	22.9 ± 13.5	1.3 ± 0.1	17.8 ± 3.1	23.3 ± 3.2
150-k	13.3 ± 5.6	57 ± 2.6	5.4 ± 1.2	55.5 ± 22.7	5.9 ± 0.7	27.5 ± 2.4	10.5 ± 3.0

R-5-P+ADP+P_i-dependent CO₂ fixation activity: sequential activities involved in this assay are Electron transport chain, CF₁, RPI, PRK, and RuBisCO. The linked activity is expressed as μmoles of CO₂ fixed, mg chlorophyll⁻¹, h⁻¹. The controls without ADP and DTT had specific activities in the range of 0.04 to 0.06 μmoles of CO₂ fixed, mg chlorophyll⁻¹, h⁻¹ for all the fractions.

R-5-P-dependent GAPDH activity: the sequential activities involved in this assay are RPI, PRK, RuBisCO, PGK, and GAPDH. The linked activity is expressed as μmoles of NADPH⁺ oxidized, mg chlorophyll⁻¹, h⁻¹. The NADPH reduction in absence of R-5-P was used as blank.

All the values represent mean ± SE (n = 3).