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In-Vitro Kinase Assay with Ser228 Mutants of SOS2CA.
GST-fused SOS2CA, SOS2CA S228A, or SOS2CA S228D was incubated with [γ-32P] ATP and separated with SDS–PAGE, followed by Coomassie staining (A) and autoradiography (B). Autoradiograph of Thrombin protease-digested SOS2CAs is shown (C). Expected sizes of SOS2CA and GST are indicated by arrow and arrowhead, respectively.
