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. 2008 Dec 9;2(1):43–58. doi: 10.1093/mp/ssn081

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© The Author 2008. Published by the Molecular Plant Shanghai Editorial Office in association with Oxford University Press on behalf of CSPP and IPPE, SIBS, CAS.

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Figure 3. — Plants Overexpressing MYB62 Have Altered Morphology, Physiology, and Development.

(A) Phenotype of MYB62 overexpressing (35S:M62-1, 35SM62-2) and GFP::MYB62 overexpressing (35S::GFP::M62) plants relative to wild-type (WT) plants. Plants were germinated and grown on soil for 3 weeks.

(B) Phenotype of older MYB62 overexpressing plants at various stages of bolting. Plants grown on soil are shown left to right in the panel.

(C) Total chlorophyll content in two independent MYB62 overexpressing transgenic lines (M1 and M2) relative to wild-type (WT). Chlorophyll content was estimated in leaf discs from 3-week-old soil-grown plants. Values are mean ± SE (n = 4).

(D) Total anthocyanin content in wild-type (WT) and MYB62 overexpressing plants (M62) grown hydroponically under Pi-sufficient (P+) and Pi-deprived (P–) conditions. Values are mean ± SE (n = 6).

(E) Germination is delayed in MYB62 overexpressing (M1 and M2) transgenic lines as compared to wild-type (WT). The germination rate was scored after the seeds were sown on peat and vernalized for 4 d.

(F) Leaf initiation is delayed in MYB62 overexpressing (M1 and M2) transgenic lines as compared to wild-type (WT). The emergence of the first rosette leaf was recorded.

(G) MYB62 overexpressing (M1 and M2) transgenic lines have significantly more rosette leaves. Number of rosette leaves was recorded after 15, 20, 25, 30, 35, and 40 d of growth on soil medium.

(H) Bolting is delayed in MYB62 overexpressing (M1 and M2) transgenic lines. Time of appearance of the first inflorescence stem was recorded in plants grown on soil medium. Values in panels (E)–(H) are mean ± SE (n = 25).