HOS3 Contitutively Regulates Stomatal Aperture Under Well Watered Conditions and Mediates ABA-Induced Stomatal Closure.
(A) Water loss of intact 4-week-old hos3-1 (closed circles) seedlings was determined gravimetrically and compared with C24 (RD29:LUC) wild-type (open circles). Means and standard errors were determined by ANOVA with a confidence interval of α = 0.05 for n = 12. Error bars represent ±1 SE.
(B) C24 (RD29A:LUC) wild-type (open circles) and hos3-1 mutant (closed circles) shoots previously untreated or (C) treated with ABA (50 μM) were excised for gravimetric water loss determination, expressed as a percent of original fresh weight.
(D) Data from (B) and (C) were plotted to express differential response in C24 (RD29A:LUC) wild-type (open circles) or hos3-1 (closed circles) when treated with ABA.