Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Jan;2(1):138–151. doi: 10.1093/mp/ssn085

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author 2009. Published by the Molecular Plant Shanghai Editorial Office in association with Oxford University Press on behalf of CSPP and IPPE, SIBS, CAS.

PMC Copyright notice

Figure 4. — HOS3 Expression Analysis and Confirmation of the hos3-2 Phenotypes.

(A) Physical map of T-DNA insertion in the hos3-1 (open) and hos3-2 (shaded) mutant alleles (Accession #At4g36830).

(B) Expression of HOS3 was determined by RT–PCR using HOS3 gene-specific primers and 1 μg total RNA from C24 (RD29A:LUC) (C24), hos3-1 (h3-1), Columbia-0 (Col), Col-0 plants overexpressing HOS3 (OE), and hos3-2 (h3-2). Tubulin (TUB) was used as a loading control.

(C) hos3-2 confirms HOS3 function in osmotic stress response in vitro and in constitutive regulation of stomatal aperture under well watered conditions. Root elongation of Col-0 (open bars) and hos3-2 (closed bars) transferred to media containing NaCl (0, 50, 100, or 150 mM). Means and standard errors were determined using ANOVA with a confidence interval of α = 0.05. Error bars represent ±1 SE.

(D) Root elongation of Col-0 (open bars) or hos3-2 (closed bars) transferred to media containing ABA (0, 1, 3, or 10 μM).

(E) Water loss of intact 4-week-ld hos3-2 (closed bars) seedlings was determined gravimetrically and compared with Col-0 (open bars) wild-type. Means and standard errors were determined by ANOVA with a confidence interval of α = 0.05 for n = 12. Error bars represent ±1 SE.