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. 2009 Jan;2(1):138–151. doi: 10.1093/mp/ssn085

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© The Author 2009. Published by the Molecular Plant Shanghai Editorial Office in association with Oxford University Press on behalf of CSPP and IPPE, SIBS, CAS.

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Figure 5. — HOS3 Functions as a Negative Regulator of the RD29A Stress Response Pathway as 35SCaMV:HOS3 Restores Wild-Type Sensitivity to NaCl-Stress.

(A) HOS3 restores wild-type RD29A:LUC luminescence to hos3-1. Sensitivity to stress was determined by examining changes in luminescence of untreated hos3-1 (light grey), C24 (RD29A:LUC) (dark grey), and 35S HOS3:hos3-1 (medium grey) plants compared with plants after treatment with 300 mM NaCl. Means and standard errors were determined by ANOVA with a confidence interval of α = 0.05. Error bars represent ±1 SE.

(B) HOS3 restores wild-type root growth osmotic stress sensitivity to hos3-1. hos3-1 root growth (dark grey bars) is tolerant to all concentrations of NaCl tested (0, 50, 100, and 150 mM) compared with wild-type (open bars), whereas plants expressing the 35SCaMV:HOS3 (light grey bars) showed restoration of wild-type sensitivity to NaCl. Means and standard errors were determined by ANOVA with a confidence interval of α = 0.05. Values are means ±1 SE.

(C) Root growth of hos3-1 (dark grey bars) was also compared with growth of plants expressing a 35SCaMV:HOS3 (light grey bars) and wild-type (open bars) after 6 d on media containing ABA (0, 1, 3, and 10 μM).