Figure 6.

HOS3 Complements elo2 and elo3 Monensin-Sensitive Phenotype.

WT or elo mutant yeast cells were transformed with the PYPGE15 vector containing the plant HOS3 ORF. Serial dilutions of cells were spotted onto selective media containing 0 μg monensin (left panel) or 100 μg monensin (right panel) for 10⁴, 10³, 10², or 10¹ cells, respectively, from left to right for each panel shown. Colony growth was compared after 30°C incubation for 4 d. From top to bottom, colonies represent WT vector transformed cells (WT_v), elo2 + vector (elo2_v), elo2 + HOS3 (elo2_v:HOS3), elo3 + vector (elo3_v), elo3 + HOS (elo3_v:HOS3).