Table 5.
Construct | Spikelet fertility (%) |
Relative spikelet fertility |
|||
Normal growth | Drought stress in field | Drought stress in PVC | Drought stress field | Drought stress PVC | |
Wild-type (ZH11) | 76.7 ± 1.1 | 34.4 ± 1.0 | 58.7 ± 3.7 | 0.45 | 0.77 |
Actin1:CBF3 | 62.5 ± 2.8** | 21.2 ± 1.0* | 53.6 ± 3.3 | 0.34 | 0.86* |
HVA22P:CBF3 | 61.5 ± 3.0** | 15.7 ± 1.2** | 43.8 ± 3.0** | 0.26* | 0.89** |
Actin1:SOS2 | 62.0 ± 1.4** | 30.1 ± 1.4 | 50.6 ± 3.3 | 0.49 | 0.82 |
HVA22P:SOS2 | 62.3 ± 1.1** | 48.4 ± 1.2** | 46.3 ± 3.2* | 0.78** | 0.74 |
Actin1:NCED2 | 61.7 ± 1.3** | 13.5 ± 1.1** | 45.5 ± 4.0* | 0.22* | 0.73 |
HVA22P:NCED2 | 60.0 ± 1.6** | 21.2 ± 1.0* | 47.6 ± 3.1* | 0.35 | 0.79 |
Actin1:NPK1 | 64.4 ± 2.4* | 13.6 ± 0.7** | 59.7 ± 3.2 | 0.21* | 0.82 |
HVA22P:NPK1 | 64.7 ± 1.1* | 26.4 ± 1.2 | 55.7 ± 3.8 | 0.41 | 0.72 |
Actin1:LOS5 | 64.3 ± 1.5** | 26.1 ± 1.0 | 53.6 ± 3.5 | 0.41 | 0.83* |
HVA22P:LOS5 | 68.3 ± 1.9* | 26.3 ± 1.5 | 56.1 ± 4.3 | 0.39 | 0.83* |
Actin1:ZAT10 | 70.4 ± 2.7 | 40.8 ± 1.6* | 60.4 ± 3.2 | 0.58* | 0.86* |
HVA22P:ZAT10 | 61.9 ± 2.8* | 27.0 ± 1.1 | 60.2 ± 3.7 | 0.44 | 0.97** |
Actin1:NHX1 | 68.7 ± 1.8* | 23.0 ± 0.9** | 57.3 ± 2.9 | 0.33 | 0.93* |
HVA22P:NHX1 | 69.5 ± 1.7 | 19.9 ± 1.2** | 50.2 ± 3.9* | 0.29* | 0.86* |
The value is mean ± SE (n = 30 and 10 families for treatments in field and PVC, respectively). For each family, 16 and 10 plants were measured for treatments in the field and PVC, respectively.
** and * indicate significant difference (by LSD test) between transgenic families from each construct and the wild-type at the probability level of P = 0.01 and P = 0.05, respectively. Significantly higher values in transgenic rice are highlighted in bold.