Figure 4.

The Formation of Late Hsf-HSE Binding Complexes in Wild-Type and hsfB1/hsfB2b Plants.

Leaves of Arabidopsis wild-type (WT) (Col-0) and double Hsf mutant (hsfB1/hsfB2b) plants were incubated at 37°C for different periods of time (0, 10, 30, 60, 120, and 240 min). Total protein was extracted from each sample and subjected to EMSA using radioactive-labeled synthetic HSE consensus sequence as a probe. The first lane contains only the free probe without protein. The major late Hsf–HSE binding complex (Lohmann et al., 2004) is marked by an arrowhead; the unspecific constitutive binding complexes (Lohmann et al., 2004) are marked by asterisks.