Table 3.
Comparison of Gene Expression Levels Determined by Gene Chip Hybridization and qRT–PCR.
| Gene | WT (C) |
WT (HS) |
hsfB1/B2b (C) |
hsfB1/B2b (HS) |
||||
| RT–PCRa | GCb | RT–PCRa | GCb | RT–PCRa | GCb | RT–PCRa | GCb | |
| Pdf1.2a | 1.3 | 0.9 | 2.3 | 2.0 | 39.1 | 23.7 | 47.6 | 37.4 |
| Pdf1.2b | 1.4 | 2.1 | 2.1 | 4.2 | 36.2 | 34.4 | 61.8 | 65.1 |
| AtHsfA2 | 1.8 | 1.2 | 1965.4 | 459.5 | 7.6 | 5.3 | 1922.9 | 475.7 |
| DnaJ | 1.3 | 0.9 | 267.1 | 202.8 | 2.9 | 2.7 | 293.1 | 215.8 |
| Atmkk7 | 1.7 | 0.6 | 0.0 | 0.2 | 0.0 | 0.1 | 0.0 | 0.2 |
| AtHsfB1 | 12.9 | 2.7 | 238.6 | 79.6 | 0.5 | 0.1 | 0.4 | 0.3 |
| AtHsfB2b | 0.1 | 0.3 | 3.0 | 26.2 | 0.0 | 0.2 | 0.0 | 0.3 |
| Stp3 | 16.2 | 9.7 | 0.7 | 0.4 | 18.2 | 10.4 | 1.2 | 1.3 |
| F7H2.2 | 16.9 | 36.1 | 0.7 | 1.6 | 22.4 | 49.7 | 1.1 | 4.2 |
a
mRNA level determined quantitative PCR. Relative amounts were calculated and normalized with respect to Act2 mRNA (= 100%). Data represents mean ± range (n = 2).
b
Expression estimates based on the gene chip (GC) analysis; data represent normalized and averaged intensity values of the replicates relative to the signal of Act2 mRNA hybridization (= 100%).