Table 6.
Double Mutations Identified with T-DNA Left Border Primer and the Gene-Specific Primers.
AtHsfB1 (At36990) | 5′-AAAAGTTCGCCGGAGATGACG-3’ |
5′-GTCGCAACCTTCGCACTCACT-3’ | |
AtHsfB2b (At11660) | 5′-CACAGAGGTCAATTCCGACGC-3’ |
5′-CTTCTTCCTCTGCAGCACCCA-3’ | |
T-DNA (Lba1) | 5′-ATGGTTCACGTAGTGGGCCATC-3’ |