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Requirement of elevated Smurf expression for the migration and invasion of MDA-MB-231 cells. Transient transfection of siRNAs was used to attenuate Smurfs expression in MDA-MD-231 cells. A, immunofluorescent staining of F-actin showing disruption of cell protrusion as the result of interfering with Smurfs expression. Scale bar, 5 μm. B and C, cell migration and invasion assays in transwell chambers. Scale bar, 10 μm.
