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. 2008 Sep 8;107(2):416–426. doi: 10.1093/toxsci/kfn192

FIG. 6.

FIG. 6.

RNAi-induced knockdown of GS expression in S2 cells. (A) dsRNA was targeted to the 5′ region of CG6835 and CG32495 and cell viability measured under differing concentrations of arsenite-supplemented growth medium. *p < 1.0 × 10−6 for 35 and 45μM As WT versus 35 and 45μM As GS RNAi. (B) RT-PCR analysis of GS transcript levels after targeting the 5′ region. Results have been normalized to actin. *p < 0.01. (C) dsRNA was targeted to the 3′ region of CG6835 and CG32495 and viability measured as described. *p < 1.0 × 10−7 for 35 and 45μM As WT versus 35 and 45μM As GS RNAi. (D) Real-time RT-PCR analysis of GS transcript levels after targeting the 3′ region. Results have been normalized to actin. *p < 0.01.