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. 2008 Aug 20;28(34):8430–8441. doi: 10.1523/JNEUROSCI.2752-08.2008

Figure 1.

Figure 1.

Aa–Al, NeuN immunostaining and Fluoro-Jade B staining of representative coronal brain sections at the level of the dorsal hippocampus at 7 d after reperfusion from sham, placebo (Pla)- and E2-treated female ovariectomized rats subjected to global cerebral ischemia. Global ischemia induced significant neuronal degeneration and neuronal cell loss in the CA1 pyramidal cell layer, with little or no cell loss apparent in CA3 or DG. E2 treatment afforded nearly complete protection of the hippocampus CA1 from global cerebral ischemia-induced neuronal degeneration and neuronal cell loss. Boxed areas in the left column are shown at higher magnification in right column (magnification, 40×). Scale bar, 50 μm. B, Neuronal counts of NeuN-positive neurons. Counts refer to CA1 neurons of sham-, placebo-, and 17β-estradiol-treated ovariectomized rats. Note the significant neuroprotection by E2. Values are mean ± SEM of determinations from seven individual rats. # p < 0.01 versus placebo.