Southern blot analysis of somatic cell hybrids
using C1 as a probe. A3 and A9 are the hamster and mouse controls, X
and 1 the hybrids containing these chromosomes as the only human
component, and dX and d1 the CL89-12117-derived somatic cell hybrids
containing either der(X) or der(1). RCC1 (CL89-12117), RCC2
(CL89-17872), and REN11-TT are the t(X;1)-positive RCCs. Lane REN11-N,
normal tissue adjacent to REN11-TT; and lane NK, normal kidney tissue.
HL60 is a myeloid leukemia cell line. As a molecular size marker, λ
HindIII DNA was used. DNA samples were cleaved with
EcoRI.