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. 1996 Dec 24;93(26):15299–15304. doi: 10.1073/pnas.93.26.15299

Figure 4.

Figure 4

Coimmunoprecipitation of the KRAB-A domain from two distinct zinc finger proteins and KRIP-1.2. (A) COS cells were cotransfected with pMT3-KRIP-1.2, encoding the HA-tagged KRIP-1.2 with pMFH-Kid-1N, wild-type pMFH2, or pMFH2-Kid-1A, which encode T7–GAL4 fusion proteins with the non-zinc-finger region of Kid-1, no additional sequence, or the KRAB-A region of Kid-1 respectively. After cell lysis, preparation of soluble (S) and pellet (P) fractions, immunoprecipitation with the anti-HA antibodies, SDS/PAGE resolution of the S, P, and immunoprecipitate fractions (IP), transfer to an Immobilon-P membrane, and reaction with anti-T7 flag antibody, it is apparent that Kid-1N and Kid-1A immunoprecipitate with KRIP-1.2. (B) pMT3-KRIP-1.2 was cotransfected with pBXG1 constructs encoding GAL4 fusion proteins of Kid-1N, Kid-1A, and the KRAB-A region of ZNF2. Kid-1N and the KRAB-A regions of Kid-1 and ZNF2 coimmunoprecipitate with KRIP-1.2 when the complexes are precipitated by anti-HA antibody. The blot is stained with anti-GAL4 antibody.