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. 1996 Dec 24;93(26):15305–15310. doi: 10.1073/pnas.93.26.15305

Figure 3.

Figure 3

Induction of nodule formation on M. sativa cv. Europa by living bacterial cells of R. tropici CFN299 carrying various combinations of R. meliloti nod genes. (A) R. meliloti, Rm2011; R. tropici, Rt299; R. tropici (pGMI149), Rt (pGMI149); R. tropici (pGMI149)(pGMI1962), Rt hyb. (B–D) R. tropici (pGMI149)(pGMI1962) with Tn5 insertions in the R. meliloti nod genes carried in pGMI149. (B) Mutations in the sulfation genes nodP, nodQ, and nodH. (C) Mutations in the nodFE and nodL genes. (D) Mutations in the nodA, nodB, nodC, and nodIJ genes. Rt hyb, R. tropici (pGMI149)(pGMI1962); Rt hyb D1-, R. tropici (pGMI149nodD1)(pGMI1962) and so on. Nodule numbers represent the average of 20 tubes, each containing two seedlings. Statistical analysis of nodule numbers 2 weeks after inoculation: treatments with letters in common do not differ significantly at the P = 0.05 level. Analysis of variance with Fisher’s test.