Negative ion MALDI mass spectra of NFs from the
following R. tropici (pGMI149)(pGMI1962) derivatives.
(A) The control strain with unmodified pGMI149.
(B) A derivative with a
nodC::Tn5 insertion in pGMI149.
(C) A derivative with a
nodI::Tn5 insertion in pGMI149.
Tetrameric (IV) and pentameric (V) NFs were found with the following
substitutions (nomenclature as proposed in ref. 9). 1-IV: m/z 1105 (IV,
C16:0,S), m/z 1103 (IV, C16:1,S); 2-IV: m/z 1131 (IV, C18:1,S); 3-IV:
m/z 1147 (IV, Ac, C16:0,S), m/z 1145 (IV, Ac, C16:1,S), m/z 1145 (IV,
Me, C18:1,S); 4-IV: m/z 1173 (IV, Ac, C18:1,S); 1-V: m/z 1308 (V,
C16:0,S), m/z 1306 (V, C16:1,S); 2-V: m/z 1334 (V, C18:1,S); 3-V: m/z
1350 (V, Ac, C16:0,S), m/z 1348 (V, Ac, C16:1,S), m/z 1348 (IV, Me,
C18:1,S); 4-V: m/z 1376 (V, Ac, C18:1,S); 5-V: m/z 1390 (V, Me, Ac,
C18:1,S).