Table 1.
Bacteria and plasmids used in this study
| Designation | Relevant characteristics | Ref./source |
|---|---|---|
| R. meliloti | ||
| GMI51 | RCR2011 = SU47, wild type, Nod+, Fix+ on M. sativa | 23 |
| R. tropici | ||
| GMI9776 | CFN299, wild type, NalR, Brazil | 24 |
| GMI3067 | CFN299 (pGMI149)(pGMI1962) | This study |
| GMI3066 | CFN299 (pGMI1962) | This study |
| Plasmids | ||
| pGMI149 | pRK290 prime (IncP), TcR | 25 |
| pGMI1962 | = pnodL, pMP190-prime (IncQ), CmR, | 12 |
| pGMI353 | pGMI149 nodA::Tn5#2208 | 25 |
| pGMI318 | pGMI149 nodB::Tn5#154 | 25 |
| pGMI362 | pGMI149 nodC::Tn5#2217 | 25 |
| pGMI377 | pGMI149 nodI::Tn5#2111 | 25 |
| pGMI1984 | pGMI149 nodJ::Tn5#2412 | 25 |
| pGMI41589 | pGMI149 nodP::Tn5 | 9 |
| pGMI306 | pGMI149 nodQ::Tn5#115 | 25 |
| pGMI336 | pGMI149 nodH::Tn5#2313 | 25 |
| pGMI5625 | pGMI149 Δ(nodF)6 | 13 |
| pGMI334 | pGMI149 nodE::Tn5#2309 | 25 |
| pGMI930 | pGMI149 nodD1::Tn5 | 26 |
| pGMI860 | pGMI149 nodD3::MudII lac#V40 | 26 |
| pMH682 | pWB5a-prime (IncP), 8-kb insert carrying R. meliloti nodD3 and syrM, TcR | 14 |
| pXLGD4 | pGD499-prime (IncP) carrying a hemA–lacZ fusion, TcR | 27 |
| pRK2013 | Helper plasmid for mobilization of IncP and IncQ plasmids, KmR | 28 |