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Expression of profibrotic genes in WT and ROCK1+/− mice. Hearts were isolated from mice receiving saline or Ang II for 4 weeks. A, CTGF mRNA expression was analyzed by Northern blotting. Expression levels of CTGF mRNA were standardized by expression levels of GAPDH mRNA. n=8 in each group. B, Expression of TGFβ and collagen type III mRNAs was analyzed by real-time PCR. Values were standardized by expression levels of GAPDH mRNA. n=4 in each group.
