Cultured neurons were treated with either medium (control), PACAP38 (100 nM), thrombin (100 nM) or treated with thrombin and later exposed to PACAP38 (thrombin + PACAP38). Cultures were incubated for 24 h, washed, cells collected and lysates analyzed for Bcl-2 expression by western blot analysis. Histogram represents densitometric measurements of three western blot experiments normalized to GAPDH levels. Treatments were compared by one-tailed t-test.
** p< 0.01 versus control