Figure 9. PACAP38 increased formation of active p57^Kip2 in thrombin treated neurons.

Cultured neurons were treated with either medium (control), PACAP38 (100 nM), thrombin (100 nM) or treated with thrombin and later exposed to PACAP38 for 1 h. Cultures were incubated for 24 h washed, cells collected and lysates analyzed for p57^Kip2 expression by western blot analysis. Histogram represents densitometric measurements of three western blot experiments normalized to GAPDH levels. Treatments were compared by one-tailed t-test.

***p<0.001 vs control

^a p<0.001 vs thrombin