Table 3.

Reactivity of 3Ob.1 to a panel of MBPp85-99 peptide antigens

Antigen	Peptide	Antigen, cpm	IL-4 secretion, pg/ml	IL-5 secretion, pg/ml	IL-10 secretion, pg/ml	IFN-γ secretion, pg/ml
No Antigen	—	1,746	0	0	0	0
Native	HFFK	81,714	1,676	10,500	0	83
90 substitutions	AFFK	2,125	0	0	0	0
	FFFK	5,020	0	280	0	0
	KFFK	3,130	0	0	0	0
91 substitutions	HYFK	3,706	0	290	0	0
	HAFK	2,898	0	290	0	0
	HLFK	3,585	0	0	0	0
	HHFK	3,349	0	0	0	0
	HWFK	2,952	0	0	0	0
93 substitutions	HFFR	1,907	0	0	0	0
	HFFA	15,361	196	2,400	0	0
	HFFL	1,633	0	0	0	0
	HFFH	1,671	0	0	0	0
91 Y/93X substitutions	HYFH	95,305	2,349	11,000	0	0
	HYFL	2,537	0	280	0	0
	HYFA	19,324	243	2,400	0	0
93 R/91X substitutions	HYFR	50,962	740	6,800	0	0
	HAFR	83,858	972	10,000	0	0
	HLFR	71,215	1,049	10,000	0	0
	HHFR	88,287	1,604	11,000	0	0

A representative of two experiments using 3Ob.1 is shown. Standard errors were <10%. T cells were tested for their reactivity to peptides at a concentration of 50 μM.