Table 4.

Complementary mutations

Clone	FP′1 (93)		FP′2 (91)
	ENPVVHFFRNIVTPR*		ENPVVHFFRNIVTPR
Ho.A226	R	⇒	F
	K	⇒	Y,L,H
	K	⇐	Y
	R	⇐	F
	ENPVVHFFKNIVTPR†		ENPVVHFFKNIVTPR
Hy.152	K	⇒	F
	R	⇒	Y,H,A
	K	⇐	F
	R,L	⇐	Y
3Ob.1	K	⇒	F
	R	⇒	Y,L,H,A
	K	⇐	F
	H,R	⇐	Y

The table summarizes the allowable amino acid residues that are able to activate T-cell clones >30% of the original stimulating peptide. The arrows point to which amino acids are allowed in one FP given the corresponding amino acid in the other FP. 

^*Ho.A226 was generated against MBPp85-99 (93R) peptide (HFFR). 

^†Hy.152 and 3Ob.1 were generated against MBPp85-99 peptide (HFFK), the native sequence of MBP.