Skip to main content
. 2008 Dec 2;18(5):797–808. doi: 10.1093/hmg/ddn406

Figure 1.

Figure 1.

Generation and expression of the C150S-Rds transgene. (A) Schematic diagram of C150S transgene constructs. The transgene is composed of the full-length mouse RDS cDNA with the C150S mutation followed by an SV40 poly-A tail and directed either to rods by the MOP or to cones by the human red/green opsin promoter (COP). (B) Northern blot analysis of whole retinal RNA extracts (10 µg/sample) taken from 1-month-old MOP-T transgenic, non-transgenic WT littermates and rds+/− mice. Two different probes were hybridized to the membrane: one from exon 1 of the RDS cDNA that recognizes both endogenous and transgene transcripts (left) and an SV40 poly-A probe that recognizes the transgenic message only (right). (C) qRT–PCR was used to measure levels of total RDS message from WT, rds−/−, MOP-T/rds−/− and COP-T/rds−/− retinas. Analysis was performed on retinas from 3- to 4-week-old mice using primer specific to the endogenous RDS gene.