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. Author manuscript; available in PMC: 2010 Jan 30.
Published in final edited form as: J Neurosci Methods. 2008 Sep 11;176(2):96–103. doi: 10.1016/j.jneumeth.2008.08.035

Figure 2.

Figure 2

Determining transfection efficiency. In each nerve specimen, transfected Schwann cells were counted in two parallel, longitudinal sections that lay 90u on either side of the nerve diameter. Counts were made of both transfected and non-transfected Schwann cells that were intersected by a line through the zone of maximum expression, the area with the largest number of labeled Schwann cells (usually at the sight of injection), and by two lines placed 250u proximal and distal to this line.