Figure 1.

Nucleotide and predicted amino acid sequence of 1.9-kb TCRγ G186cDNA (GenBank accession no. U22666U22666). Boundaries between the leader peptide (L), variable (V), junctional (J), extracellular (Cex), transmembrane (TM), and cytoplasmic (Cyt) regions are indicated by arrow heads. Boundary and canonical residues are numbered according to mammalian Vγ gene segment convention (6, 7). The 51-residue leader peptide was predicted by the psort program (http://psort.nibb.ac.jp) based on the criteria of von Heijne (19). Putative CDR1 and CDR2 regions are indicated. Asp₁₁₇ is the first position assigned to the Cγ region, although in TCR γ-chains using Jγ3 the aspartic acid is replaced by an asparagine (Fig. 3B). Residues conserved in mammalian Cγ genes are underlined. One potential N-glycosylation site (★) is found at position 237. A consensus polyadenylylation sequence is boldfaced.