Figure 3.

TCR Vγ and Jγ nucleotide and amino acid sequences. (A) Comparison of amino acid sequences of Vγ1, Vγ2, and Vγ3 subfamily members characterized according to sequence data of genomic gene segments, cDNA clones from the cDNA library, and from anchored-PCR or specific PCR. Residues identical to the prototypic sequence are indicated by dashes. Vγ1.1b, Vγ1.3b, and Vγ2.5 are partial amino sequences. (B) Nucleotide sequences of Vγ–Jγ junctions. Vγ usage is indicated on the left and Jγ usage on the right. Vγ, Jγ1, and Jγ2 gene segment assignment is based on germ-line sequences. Dashes indicate identity to the germ-line Vγ sequence. Assignment of nucleotides to the Jγ3 gene segment is based on a consensus sequence of TCR γ-chains using the Jγ3 segment. Nucleotides that cannot be assigned to either Vγ or Jγ gene segments are indicated as N- or P-additions. Putative P nucleotides are underlined. (C) Recombination signal sequences of Vγ1, Vγ2, Vγ3, Jγ1, and Jγ2 gene segments. Heptamer/nonamer sequences, indicated in boldface type, were identified by comparison to the consensus sequences of mammalian TCR/Ig genes (27). Positions matching the consensus sequences are underlined.