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. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: J Mol Cell Cardiol. 2008 Sep 30;46(1):4–14. doi: 10.1016/j.yjmcc.2008.09.707

Figure 5. NO scavengers blunt the protective effect of NIR.

Figure 5

Neonatal myocytes were subjected to HR with or without treatment with NIR (7.5 J/cm2) in the presence of NO scavengers carboxy-PTIO or oxyHb, or NOS inhibitor L-NMMA. (A) LDH release into the medium and (B) caspase 3 activity were measure as described under Material and Methods. NO scavengers abolished protective effect of NIR completely and L-NMMA only partially. Addition of sodium nitrite (NO2−) in the presence of L-NMMA restored the NIR induced protection to control LDH-release values. Values for LDH or Caspase 3 activity were expressed as % of control which was set as 100%. * p<0.01 vs. control, # p<0.05 vs. HR (no intervention), n=6.