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Analysis of B-RAF/MEK/ERK activation in tissues derived from LSL-B-rafV600E ± Mx1-Cre mice. Protein lysates were harvested from various tissues and subjected to (A) B-Raf kinase assays and (B) Western blot analysis with antibodies for phospho-ERK, phospho-MEK, and cyclin D1. Immunoblotting with a total ERK antibody was used to confirm protein loading.
